电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

概要 ：探究紫外线是否可通过裂解生长因子-β（TGF-β）-依赖性的上皮-脂质转换 (EMT)来倡导恶性肿瘤的洪水泛滥搬迁。使用总和2Gy(60)Coγ线照射源自人类器官的6种恶性肿瘤，日志与EMT相关的叠加，这除此以外分别并用显微镜技术，细胞内质印迹方律，免疫荧光技术，划痕试验和Transwell小室试验来推论并检测细胞一组织形态，EMT记号，洪水泛滥搬迁控制能力等。采用酶六轮免疫吸附律检测这些恶性肿瘤中都TGF-β细胞内高度，并用值得注意依赖性剂SB431542来评估TGF-β接收器通路在紫外线EMT中都的作用。经过总和为2Gy照射的恶性肿瘤中都假定间叶细胞的表达，与实为照射一组相比其上皮记号下降，间叶细胞记号增加，同时其洪水泛滥集中都于控制能力提升，TGF-β细胞内高度也提高。实质性发现由A549紫外线游离的EMT可通过对TGF-β接收器依赖性发生反败为胜。这些结果表明TGF-β依赖性的EMT在紫外线游离提升恶性肿瘤洪水泛滥集中都于控制能力中都起着关键作用。

主笔： lizexiu